Bench Talk

Stephanie Sandor

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Two Trending CRISPR-Cas9 Optimizations

Posted by Stephanie Sandor on Jan 24, 2019 1:20:02 PM

The spotlight continues to shine on an innovative technology, CRISPR-Cas9, due to its efficient site-directed mutagenesis. An example includes introducing variant Cas9 nucleases which affect the product of the studied protein. Also, it is utilized for RNA editing as opposed to DNA editing. The traditional use for Cas9 nuclease introduces double-strand DNA breaks that are repaired by nonhomologous end joining (NHEJ) or homology-directed repair (HDR) mechanics. These cause spontaneous insertions or deletions (InDels) which may give unwanted phenotypes.

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Tags: CRISPR-Cas9